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Biology Revision Guides

B3: Respiration, Photosynthesis and Bioenergetics

Year 1 / ASYear 2 / A-Level All Boards (AQA, Edexcel, OCR, WJEC, CCEA) AQA

Glycolysis, Krebs cycle, oxidative phosphorylation, light-dependent and light-independent photosynthetic reactions.

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📋 Key Definitions and Core Concepts

Chemiosmosis: ATP synthesis driven by proton electrochemical gradient across inner mitochondrial/thylakoid membrane through ATP synthase.
Photolysis: Light-dependent splitting of water in PSII: 2H₂O → 4H⁺ + 4e⁻ + O₂.

🔍 Key Principles & Specification Requirements

  • Glycolysis: glucose → 2 pyruvate, net 2 ATP, 2 NADH (cytoplasm).
  • Krebs cycle: acetyl-CoA oxidized producing 2 CO₂, 3 NADH, 1 FADH₂, 1 ATP per turn (mitochondrial matrix).
  • Calvin cycle (stroma): RuBisCO fixes CO₂ to RuBP (5C) → 2 GP (3C) → reduced to 2 TP (3C) using ATP and NADPH.

💡 Worked Example Question

Exam-Style Question

Question:

Explain why oxygen is essential for aerobic ATP production.

Model Solution & Mark Scheme:

Oxygen acts as the terminal electron acceptor at complex IV of the electron transport chain. It combines with electrons and protons to form H₂O (½O₂ + 2H⁺ + 2e⁻ → H₂O), maintaining electron flow and proton gradient across the inner mitochondrial membrane.

❓ Practice Questions & Mark Schemes

Q1: What happens to the Calvin cycle in prolonged darkness?

Show Model Answer

Answer: Without light-dependent ATP and NADPH, GP cannot be reduced to TP. GP accumulates while RuBP and TP levels drop, halting glucose synthesis.

Q2: Calculate the respiratory quotient (RQ) for tripalmitin C₅₁H₉₈O₆ + 145/2 O₂ → 51 CO₂ + 49 H₂O.

Show Model Answer

Answer: RQ = CO₂ produced / O₂ consumed = 51 / 72.5 = 0.70.

🎬 Video Resources

📄 Past Papers & Exam Resources

🔗 Further Reading & Resources

🎓 Smart Lesson (Guided)